Osmosis Jones (voice)

Drix (voice)

Mayor Paul Spryman / Hector's Mom (voice)

Hector Cruz (voice)

Maria Amino (voice)

Police Chief Gluteus (voice)

Travis Lum (voice)

Ellen Patella (voice)

Brain Advisor #1 (voice)

Dander (voice)

The Mole (voice)

General Malaise / Dr. Hammertoe (voice)

Christine Kolchuck (voice)

Rescue Cell (voice)

Mayor Santorini (voice)

Nick O'Teen (voice)

Ashley / Carbon Monoxide (voice)

Strepfinger (voice)

Cilia Tyson (voice)

Hector's Dad (voice)

Captain Quinine (voice)